Biological reagents - Bovine viral diarrhoea
Bovine viral diarrhoea is a viral disease of cattle and other ruminants caused by the Bovine Viral Diarrhoea Virus (BVDV), which is a member of the pestivirus genus.
There are four recognised species within the pestivirus genus: BVDV-1, BVDV-2, Border Disease Virus (BDV) of sheep and Classical Swine Fever Virus (CSFV).
Monoclonal antibodies for BVDV and BDV
Proficiency Testing Services are available for reagents below
Antibodies - Monoclonal (mAb) - Murine origin
Pan-pestivirus reactive monoclonal antibodies
|
mAb |
antigen |
immunoglobulin class |
detects |
|
RAE0801 |
WB103 |
NS2-3 |
IgG1 |
virtually all pestivirus |
1mg |
|
WB105 |
NS2-3 |
IgG2a |
virtually all pestivirus |
1mg |
A pool of two non-competing monoclonal antibodies, which have reacted with all pestiviruses tested at APHA to date, including BVD, CSF and BD viruses, as well as uncharacterised viruses from other ruminants. They are suitable for indirect labelling techniques for the detection of pestivirus antigens in cell cultures and tissue sections. Store at 4°C.
BVDV monoclonal antibodies
|
mAb |
antigen |
immunoglobulin class |
detects |
|
RAE2020 |
WB103 |
NS2-3 |
IgG1 |
all pestivirus |
1mg |
|
WB112 |
NS2-3 |
IgG2a |
all pestivirus |
1mg |
|
WB166 |
E2 |
IgG1 |
BVDV type 1 & BDV |
1mg |
|
WB214 |
E2 |
IgG1 |
BVD type 1 |
1mg |
A pool of four monoclonal antibodies, which detects a wider range of BVDV antigens in tissue cultures. This pool is preferable to RAE0801 for detecting bovine pestiviruses. Store at 4°C.
BVDV and BDV monoclonal antibodies
|
mAb |
antigen |
immunoglobulin class |
detects |
|
RAE0822 |
WB162 |
E2 |
IgG1 |
BVDV-1 |
1mg |
RAE0823 |
WB210 |
Erns |
IgG1 |
BVDV-1 |
1mg |
RAE0824 |
WB215 |
E2 |
IgG2b |
BVDV-1 |
1mg |
RAE4174 |
WB214 |
E2 |
IgG1 |
BVDV-1 |
1mg |
RAE0241 |
WB160 |
NS2-3 |
IgG1 |
BVDV-1 & BDV |
1mg |
RAE4173 |
WB166 |
E2 |
IgG1 |
BVDV-1 & BDV |
1mg |
RAE0993 |
WS538 |
E2 |
IgG2a |
BVDV-2 |
1mg |
RAE0992 |
WS433 |
Erns |
IgG2a |
BVDV-2 & BDV |
1mg |
RAE0825 |
WS363 |
Erns |
IgG1 |
BDV |
1mg |
RAE4751 |
WS373 |
Erns |
IgG1 |
BDV |
1mg |
RAE4750 |
WS371 |
Erns |
IgG1 |
BDV |
1mg |
Ruminant pestiviruses can be divided into BVDV1, BVDV2 and BDV. No single monoclonal antibody binds specifically to all three subtypes but the above panel taken together will recognise most strains and indicate to which subtype they belong. None of these monoclonal antibodies bind to the CSF virus, so the panel may be used in conjunction with RAE0242 or RAE0826 for the differentiation of BVD/BD and CSF isolates from pigs, as recommended by the European Community. Store at 4°C.
Further discriminatory mAbs are available on request.
References:
Edwards S, Sands J J, Paton D J (1991) Vet. Microbiol 29, 101-108.
Paton D J, Sands J J, Edwards S (1994) Arch.Virol. 135, 241-252.
Edwards S, Sands J J, Harkness J W (1988) Arch.Virol. 102,197-206.
Antisera
RAB0042 BVDV positive serum 2ml
A positive control for serum antibody assays, antigen detection procedures and within research applications. Freeze-dried, hyper-immune bovine serum derived from new born calves. Store at 4°C or at -20°C after reconstitution.
Monoclonal antibodies for CSFV
|
mAb |
antigen |
immunoglobulin class |
detects |
|
RAE0242 |
WH211 |
E2 |
IgG1 |
CSF |
1mg |
RAE0826 |
WH303 |
E2 |
IgG1 |
CSF |
1mg |
Reference:
Lin M, Mallory M, Clavijo A (2000) J Virol 74, 11619 - 11625
NB: CSFV monoclonal antibodies are not available in the UK without prior approval of Defra – please contact the Customer Service Team for further details.
To discuss your requirements please contact our Customer Services Team
Tel: +44 (0)3000 600001
Email: salesdesk@apha.gov.uk
Page last modified: 5 August, 2020